quinoline based fibroblast activation protein fap targeted tracer fapi 46  (MedChemExpress)

Journal: Theranostics

Article Title: Engineering a modular FAP-targeting ferritin-based drug nanocarrier for enhanced glioblastoma theranostics

doi: 10.7150/thno.125403

Figure Lengend Snippet: Representative chemical structures and functional groups of (A) FAPI-04, (B) FAPI-46, and (C) Alb-FAPtp-01 and (D) the developed peptide tracer (Alb-FAPtp-02). (E) FAP expression level in U-87MG and HEK293T. (F) Cellular uptake of ⁶⁸Ga-FAPI-04, ⁶⁸Ga-FAPI-46, and ⁶⁸Ga-Alb-FAPtp-02 in U-87 MG and HEK293T cells. No significant. (G) Competitive binding assay showing inhibition of tracer uptake in U-87 MG cells by non-radiolabeled FAPI-04, confirming FAP-specific binding (n = 3, mean ± s.d.). ( H ) In vivo dynamic PET imaging analysis comparison of FAP tracers. Representative dynamic PET/CT imaging of ⁶⁸Ga-Alb-FAPtp-02, ⁶⁸Ga-FAPI-46 and ⁶⁸Ga-FAPI-04 in mice with glioma xenografts. (I) the corresponding time-activity curves of radiotracers for U-87 MG tumors and muscle. Statistical significance was determined using one-way ANOVA followed by Tukey's HSD post hoc test. * p < 0.05.

Article Snippet: The FAPI-04 precursor was sourced from KriSan Biotech Co. (Tainan, Taiwan), a PICS/GMP-compliant pharmaceutical manufacturer, whereas the FAPI-46 precursor was purchased from MedChemExpress (MCE, New Jersey, USA).

Techniques: Functional Assay, Expressing, Competitive Binding Assay, Inhibition, Binding Assay, In Vivo, Imaging, Comparison, Positron Emission Tomography-Computed Tomography, Activity Assay

Journal: Biomedicines

Article Title: FAPi PET/CT Imaging to Identify Fibrosis in Immune-Mediated Inflammatory Diseases

doi: 10.3390/biomedicines13040775

Figure Lengend Snippet: FAPi PET/CT in pulmonary diseases.

Article Snippet: Rosenkrans et al., 2022 [ ] , 68Ga FAPi-46 and 68Ga-DOTA , Inveon small-animal scanner (Siemens) , 0.8–3.8 MBq for dynamic imaging and 3.8 MBq for static imaging , 0 and 60 min , HU, %IA, %IA/cc, VOI.

Techniques: Control, Comparison, Immunofluorescence, Western Blot, Biomarker Discovery, Staining, Immunohistochemical staining, Expressing, Single Photon Emission Computed Tomography, Activity Assay, Imaging, Immunohistochemistry

Journal: Biomedicines

Article Title: FAPi PET/CT Imaging to Identify Fibrosis in Immune-Mediated Inflammatory Diseases

doi: 10.3390/biomedicines13040775

Figure Lengend Snippet: FAPi PET/CT in cardiovascular, renal and IgG4-related diseases.

Article Snippet: Rosenkrans et al., 2022 [ ] , 68Ga FAPi-46 and 68Ga-DOTA , Inveon small-animal scanner (Siemens) , 0.8–3.8 MBq for dynamic imaging and 3.8 MBq for static imaging , 0 and 60 min , HU, %IA, %IA/cc, VOI.

Techniques: Control, Comparison, Activity Assay, Fluorescence, Imaging, In Vivo, Clinical Proteomics, Activation Assay, Staining, Immunohistochemical staining

Journal: Biomedicines

Article Title: FAPi PET/CT Imaging to Identify Fibrosis in Immune-Mediated Inflammatory Diseases

doi: 10.3390/biomedicines13040775

Figure Lengend Snippet: Summary of main characteristics investigated in FAPi PET/CT.

Article Snippet: Rosenkrans et al., 2022 [ ] , 68Ga FAPi-46 and 68Ga-DOTA , Inveon small-animal scanner (Siemens) , 0.8–3.8 MBq for dynamic imaging and 3.8 MBq for static imaging , 0 and 60 min , HU, %IA, %IA/cc, VOI.

Techniques: Injection, Imaging, Immunohistochemical staining, Staining, Activity Assay

Journal: Biomedicines

Article Title: FAPi PET/CT Imaging to Identify Fibrosis in Immune-Mediated Inflammatory Diseases

doi: 10.3390/biomedicines13040775

Figure Lengend Snippet: FAPi PET/CT in gastro-intestinal and hepatic diseases.

Article Snippet: Rosenkrans et al., 2022 [ ] , 68Ga FAPi-46 and 68Ga-DOTA , Inveon small-animal scanner (Siemens) , 0.8–3.8 MBq for dynamic imaging and 3.8 MBq for static imaging , 0 and 60 min , HU, %IA, %IA/cc, VOI.

Techniques: Control, Comparison, Histopathology, Immunohistochemical staining, Staining, Imaging, Diagnostic Assay, Biomarker Discovery, Immunofluorescence, Expressing